The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 25, 2014
Filed:
Nov. 27, 2003
Applicants:
Maria Keβeler, Mannheim, DE;
Thomas Zelinski, Neuleiningen, DE;
Bernhard Hauer, Fuβgönheim, DE;
Inventors:
Assignee:
BASF SE, Ludwigshafen, DE;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/63 (2006.01); C12N 15/70 (2006.01); C12N 15/74 (2006.01); C12N 1/21 (2006.01);
U.S. Cl.
CPC ...
C12N 15/63 (2013.01); C12N 15/70 (2013.01);
Abstract
The present invention relates to methods for expressing nucleic acid sequences in prokaryotic host cells, where at least one DNA construct which is capable of episomal replication in a host cell and which comprises a nucleic acid sequence to be expressed under the transcriptional control of an L-rhamnose-inducible promoter, where the promoter is heterologous with regard to the nucleic acid sequence, is introduced into the host cell and the expression of he nucleic acid sequence is induced by addition of L-rhamnose, wherein the prokaryotic host cell is at least deficient with regard to an L-rhamnose isomerase.