The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 25, 2014

Filed:

Apr. 09, 2009
Applicants:

Hans Söderlund, Espoo, FI;

Ari Hokkanen, Espoo, FI;

Kari Kataja, Espoo, FI;

Ingmar Stuns, Espoo, FI;

Kai Kolari, Espoo, FI;

Heli Siren, Espoo, FI;

Stella Rovio, Espoo, FI;

Reetta Satokari, Espoo, FI;

Jan Rautio, Espoo, FI;

Inventors:

Hans Söderlund, Espoo, FI;

Ari Hokkanen, Espoo, FI;

Kari Kataja, Espoo, FI;

Ingmar Stuns, Espoo, FI;

Kai Kolari, Espoo, FI;

Heli Siren, Espoo, FI;

Stella Rovio, Espoo, FI;

Reetta Satokari, Espoo, FI;

Jan Rautio, Espoo, FI;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12M 1/34 (2006.01); C12Q 1/68 (2006.01); G01N 33/48 (2006.01); B01L 3/00 (2006.01); G01N 33/543 (2006.01); G01N 35/00 (2006.01);
U.S. Cl.
CPC ...
G01N 33/54326 (2013.01); B01L 2200/027 (2013.01); B01L 2200/0668 (2013.01); B01L 3/50273 (2013.01); B01L 2200/10 (2013.01); B01L 3/502761 (2013.01); B01L 2200/0684 (2013.01); G01N 33/54366 (2013.01); B01L 2400/088 (2013.01); B01L 2300/0636 (2013.01); B01L 2300/087 (2013.01); G01N 35/0098 (2013.01); B01L 2300/0681 (2013.01); B01L 2300/16 (2013.01); B01L 2400/086 (2013.01); B01L 2200/0652 (2013.01);
Abstract

A microfluidic chip device (MCD) and its use for performing miniaturized assays on magnetic microbeads (MMs) are described. The MCD is particularly useful for carrying out miniaturized transcript analysis by aiding affinity capturing (TRAC) assays, including PCR. The MCD comprises at least one reaction chamber with sealable liquid connections and at least one fluidic pillar filter in each chamber. The fluidic pillar filter comprises rods with spacings allowing MMs to pass. The sealable liquid connections feed liquid to the reaction chamber, wherein air bubbles are removed. The liquid stream contacts the MMs, which are manipulated with a magnetic rod. The liquid connections enable trapping of the MMs behind the pillar filters or in the channel, while the liquid is changed.


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