The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 18, 2014
Filed:
Oct. 28, 2010
Kei Takenaka, Kashiwa, JP;
Hideki Nakamoto, Tokai, JP;
Kazuo Takei, Tokai, JP;
Masahiro Kurihara, Yokohama, JP;
Yuusuke Watanabe, Hitachinaka, JP;
Hisao Saito, Tokyo, JP;
Yasuhiko Sasaki, Tsuchiura, JP;
Kei Takenaka, Kashiwa, JP;
Hideki Nakamoto, Tokai, JP;
Kazuo Takei, Tokai, JP;
Masahiro Kurihara, Yokohama, JP;
Yuusuke Watanabe, Hitachinaka, JP;
Hisao Saito, Tokyo, JP;
Yasuhiko Sasaki, Tsuchiura, JP;
Hitachi Engineering & Services Co., Ltd., Ibaraki, JP;
Abstract
A method for accurately counting desired cells or microorganisms (viable bacteria) in a sample fluid in which contaminants are included is provided. One or plural types of membrane-permeable fluorochromes whose fluorescence amount is amplified by binding to a nucleic acid and glycerin are added to a sample fluid containing cells or microorganisms to be counted and allowed to stand for a certain time. Glycerin is added before or after or simultaneously with the mixing of the sample fluid and the fluorochrome(s). The cells or microorganisms to be counted are counted by staining the cells or microorganisms to be counted, followed by irradiating with light having a specific wavelength to detect the fluorescence emitted from the cells or microorganisms.