The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 11, 2014

Filed:

Apr. 21, 2011
Applicants:

Toshio Heike, Kyoto, JP;

Tatsutoshi Nakahata, Kyoto, JP;

Tomonari Awaya, Kyoto, JP;

Inventors:

Toshio Heike, Kyoto, JP;

Tatsutoshi Nakahata, Kyoto, JP;

Tomonari Awaya, Kyoto, JP;

Assignee:

Kyoto University, Kyoto-shi, JP;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 5/00 (2006.01); C12N 5/077 (2010.01); A61K 35/12 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0658 (2013.01); A61K 35/12 (2013.01); C12N 2506/45 (2013.01); C12N 2506/02 (2013.01);
Abstract

A method for inducing the differentiation of pluripotent stem cells into skeletal muscle or skeletal muscle progenitor cells is provided. Specifically, a method for producing artificial skeletal muscle or skeletal muscle progenitor cells from human pluripotent stem cells is provided, comprising the following steps of: (1) culturing human pluripotent stem cells by suspension culture; (2) culturing a cell population after suspension culture by adhesion culture; (3) dissociating cells after adhesion culture; and (4) culturing the dissociated cells by adhesion culture. Artificial skeletal muscle or induced skeletal muscle progenitor cells prepared by the method are also provided.


Find Patent Forward Citations

Loading…