The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 11, 2014

Filed:

Apr. 27, 2012
Applicants:

Alan L. Epstein, Pasadena, CA (US);

Melissa G. Lechner, Pasadena, CA (US);

Inventors:

Alan L. Epstein, Pasadena, CA (US);

Melissa G. Lechner, Pasadena, CA (US);

Assignee:

University of Southern California, Los Angeles, CA (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/50 (2006.01); C12N 5/078 (2010.01); G01N 33/574 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0634 (2013.01); G01N 2800/52 (2013.01); C12N 2502/30 (2013.01); G01N 33/5047 (2013.01); C12N 2503/02 (2013.01); G01N 33/5011 (2013.01); C12Q 2600/158 (2013.01); G01N 33/57484 (2013.01); C12Q 2600/106 (2013.01);
Abstract

Provided herein are methods for determining the presence of cancer (malignant versus benign), monitoring the progression of cancer, monitoring cancer relapse, monitoring the response to cancer therapy, or cancer staging in a subject, by evaluating CD33/HLA-DR, CD14/HLA-DR, CD66b/HLA-DRor, CD11b/HLA-DRMDSC for activation of a transcription factor. Transcription factors include, but are not limited to, STAT3, pSTAT3, HIF1α, or C/EBPβ. The MDSC phenotype can be CD33HLA-DRHIF1α/STAT3, CD14HLA-DRHIF1α/STAT3/pSTAT3/C/EBPb, CD66bHLA-DRHIF1α/STAT3/pSTAT3/C/EBPb, CD33HLA-DRHIF1α/STAT3/pSTAT3/C/EBPb, CD11bHLA-DRHIF1α/STAT3/pSTAT3/C/EBPb, or CD11bHLA-DRC/EBPβ. Also provided herein are methods for inducing human MDSC from healthy donor peripheral blood mononuclear cells (PBMC) by co-culturing PBMC with human solid tumor cell lines and subsequently measuring their suppressive ability.


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