The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 11, 2014
Filed:
Nov. 19, 2009
Peter Jan Leonard Mario Quaedflieg, Elsloo, NL;
Timo Nuijens, Maastricht, NL;
Claudia Cusan, Aachen, DE;
Catharina Hubertina Maria Schepers, Stein, NL;
Peter Jan Leonard Mario Quaedflieg, Elsloo, NL;
Timo Nuijens, Maastricht, NL;
Claudia Cusan, Aachen, DE;
Catharina Hubertina Maria Schepers, Stein, NL;
Enzypep B.V., Geleen, NL;
Abstract
The invention relates to a method for synthesizing a peptide by enzymatically preparing an ester or thioester from (i) an N-terminal protected amino acid or an N-terminal protected peptide where either can have a protected C-terminal ester group and (ii) an alcohol represented by the formula HO—CX—Z or a thiol represented by the formula HS—CX—Z, each X independently representing a halogen atom or a hydrogen atom; and Z represents an electron withdrawing group comprising at least one sp-hybridized carbon comprising at least two substituents comprising a heteroatom directly attached to the at least one sp-hybridized carbon or at least one sp-hybridized carbon comprising one or two substituents comprising a heteroatom directly attached to the at least one sp-hybridized carbon, and enzymatically coupling the prepared ester or thioester with an optionally C-terminal protected amino acid or with an optionally C-terminal protected peptide in a medium comprising 2 wt. % water or less.