The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 11, 2014
Filed:
Oct. 08, 2009
Sylvestre Marillonnet, Halle, DE;
Stefan Werner, Halle, DE;
Carola Engler, Halle/Saale, DE;
Romy Kandzia, Halle/Saale, DE;
Frank Thieme, Halle/Saale, DE;
Ernst Weber, Halle/Saale, DE;
Sylvestre Marillonnet, Halle, DE;
Stefan Werner, Halle, DE;
Carola Engler, Halle/Saale, DE;
Romy Kandzia, Halle/Saale, DE;
Frank Thieme, Halle/Saale, DE;
Ernst Weber, Halle/Saale, DE;
Icon Genetics GmbH, Munich, DE;
Abstract
A process of inserting a nucleic acid sequence of interest into an acceptor nucleic acid is provided. The process comprises amplifying by PCR a DNA comprising in the following order a sequence segment U, a nucleic acid sequence segment of known nucleotide sequence K2, and a nucleic acid sequence segment of known sequence K3. The process further comprises treating the linear double-stranded DNA molecules from the PCR amplification with an exonuclease to obtain a single-stranded overhang at the first end of the DNA and a single-stranded overhang comprising nucleic acid segments K2 and K3 at the second end of the DNA. The process additionally comprises annealing the product of the exonuclease treatment to a linearized double-stranded acceptor nucleic acid which has been designed to complement the single-stranded overhangs of the product of the exonuclease treatment.