The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 11, 2014

Filed:

Jun. 18, 2013
Applicant:

Ikeda Food Research Co., Ltd, Hiroshima, JP;

Inventors:

Takako Yada, Hiroshima, JP;

Koji Miyamoto, Hiroshima, JP;

Michinari Honda, Hiroshima, JP;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12M 1/00 (2006.01); C12Q 1/54 (2006.01); C12N 9/00 (2006.01); C12N 9/96 (2006.01); C12N 1/20 (2006.01); C12N 15/00 (2006.01); G01N 33/66 (2006.01); C12Q 1/32 (2006.01); C12Q 1/00 (2006.01); C12N 9/04 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/006 (2013.01); C12Y 101/9901 (2013.01); G01N 33/66 (2013.01); C12Q 1/32 (2013.01); C12N 9/0006 (2013.01); G01N 2333/902 (2013.01);
Abstract

Disclosed herein are a gene (polynucleotide) encoding an FAD-conjugated glucose dehydrogenase which can be characterized by reactivity to glucose, thermal stability, substrate-recognition performance, and low activity for maltose; a process for the production of the enzyme using a transformant cell transfected with the gene; a method for the determination of glucose; a reagent composition for use in the determination of glucose; and a biosensor for use in the determination of glucose. An embodiment is a polynucleotide encoding an FAD-conjugated glucose dehydrogenase, comprising a polypeptide whose amino acid sequence comprises X1-X2-X3-X4-X5-X6, wherein X1 and X2 independently represent an aliphatic amino acid residue; X3 and X6 independently represent a branched amino acid residue; and X4 and X5 independently represent a heterocyclic amino acid residue or an aromatic amino acid residue.


Find Patent Forward Citations

Loading…