The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 04, 2014
Filed:
Aug. 25, 2011
Adelbert Grossmann, Eglfing, DE;
Friederike Hesse, Munich, DE;
Erhard Kopetzki, Penzberg, DE;
Wilma Lau, Munich, DE;
Christian Schantz, Penzberg, DE;
Adelbert Grossmann, Eglfing, DE;
Friederike Hesse, Munich, DE;
Erhard Kopetzki, Penzberg, DE;
Wilma Lau, Munich, DE;
Christian Schantz, Penzberg, DE;
Hoffmann-La Roche Inc., Little Falls, NJ (US);
Abstract
A pro-polypeptide which is useful for the expression of a polypeptide of interest in a prokaryotic cell. Therefore the pro-polypeptide is fused to the N-terminus of the polypeptide of interest. The pro-polypeptide as reported herein provides for improved expression yields and improves the handling of the fusion polypeptide (downstream processing, purification). For example, efficient endotoxin removal is effected while the protein of interest comprising the pro-polypeptide is bound e.g. to an affinity chromatography material. Thereafter the pro-polypeptide can efficiently be cleaved from the polypeptide of interest by the incorporated protease cleavage site with the cognate protease.