The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 04, 2014

Filed:

Sep. 20, 2010
Applicants:

Dong-shan an, Daejeon, KR;

Song-gun Kim, Daejeon, KR;

Sung-taik Lee, Daejeon, KR;

Wan-taek Im, Daejeon, KR;

Chang-hao Cui, Daejeon, KR;

Inventors:

Dong-Shan An, Daejeon, KR;

Song-Gun Kim, Daejeon, KR;

Sung-Taik Lee, Daejeon, KR;

Wan-Taek Im, Daejeon, KR;

Chang-Hao Cui, Daejeon, KR;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12P 19/56 (2006.01); C12N 9/24 (2006.01); C12N 1/21 (2006.01); C12N 15/56 (2006.01); C12N 15/63 (2006.01); C12N 15/70 (2006.01); C12N 9/42 (2006.01); A61K 31/704 (2006.01);
U.S. Cl.
CPC ...
C12P 19/56 (2013.01); C12N 9/2402 (2013.01); C12Y 302/01071 (2013.01); C12Y 302/01075 (2013.01); C12Y 302/01088 (2013.01); C12Y 302/01021 (2013.01); C12N 9/2445 (2013.01); A61K 31/704 (2013.01);
Abstract

The present invention relates to Rhodanobacter ginsenosidimutans KCTC22231T-derived ginsenoside glycosidase and use thereof. The polypeptide has an activity of converting PPD (protopanaxadiol)-type saponins into in vivo absorbable and highly active deglycosylated saponins, by selective hydrolysis of a bond at a particular position of ginsenoside. The present invention also relates an amino acid sequence constituting the polypeptide, a nucleic acid sequence encoding the protein, a recombinant vector comprising the nucleic acid sequence, and a transformant transformed with the vector. The invention further provides a method for preparing Rhodanobacter ginsenosidimutans KCTC22231T-derived ginsenoside glycosidase by culturing the transformant, a method for converting PPD (protopanaxadiol)-type major ginsenoside into a rare ginsenoside that is absorbable in vivo using the protein, and a composition for converting PPD-type saponins into in vivo absorbable saponins, having the protein as an active ingredient.


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