The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 28, 2014

Filed:

Dec. 21, 2011
Applicants:

Annika Malm, Helsinki, FI;

Simo Laakso, Turku, FI;

Ossi Pastinen, Kantvik, FI;

Heidi Kahelin, Espoo, FI;

Miia Mujunen, Helsinki, FI;

Inventors:

Annika Malm, Helsinki, FI;

Simo Laakso, Turku, FI;

Ossi Pastinen, Kantvik, FI;

Heidi Kahelin, Espoo, FI;

Miia Mujunen, Helsinki, FI;

Assignee:

Neste Oil Oyj, Espoo, FI;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C10L 1/18 (2006.01); C12N 9/00 (2006.01); C12N 9/24 (2006.01); C12P 7/64 (2006.01); C12N 9/42 (2006.01);
U.S. Cl.
CPC ...
C12N 9/00 (2013.01); C12N 9/248 (2013.01); Y02E 50/16 (2013.01); Y02E 50/17 (2013.01); C12P 7/6463 (2013.01); Y02E 50/13 (2013.01); C12N 9/2437 (2013.01);
Abstract

The present invention relates to a process for producing enzymes and single cell oil. The process comprises that microorganisms capable of producing both single cell oil and enzymes are cultivated under conditions suitable for single cell oil production and enzyme production in a single cell oil production process. A microorganism culture comprising single cell oil and enzymes is obtained and at least part of the microorganism culture, of the supernatant and/or microorganism cells separated from the microorganism culture, of protein fraction enriched from the supernatant, and/or of protein fraction obtained from the cells is used as an enzyme preparation or as a source of enzymes. Single cell oil is recovered from the microorganism cells and used as biofuel, component of biofuel or as a starting material for biofuel production. Enzymes produced according to the process are used in the same or in another industrial process.


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