The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 14, 2014

Filed:

Dec. 05, 2008
Applicants:

Kenji Sawai, Kanagawa, JP;

Hideki Sawai, Kanagawa, JP;

Inventors:

Kenji Sawai, Kanagawa, JP;

Hideki Sawai, Kanagawa, JP;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 1/19 (2006.01); C12N 9/04 (2006.01); C12P 7/56 (2006.01); C12N 15/81 (2006.01);
U.S. Cl.
CPC ...
C12P 7/56 (2013.01); C12N 9/0006 (2013.01); C12N 15/81 (2013.01);
Abstract

A gene expressing cassette codes lactate dehydrogenase that is needed for prevention of deterioration in lactic acid yield and lactic acid production rate in continuous culture with simultaneous filtration of a yeast strain having a lactic acid-producing ability, which achieves high optical purity, high lactic acid yield and high lactic acid production rate simultaneously, a yeast strain having the cassette and a method of producing lactic acid by culturing the yeast strain. The lactate dehydrogenase-expressing cassette is a lactate dehydrogenase-expressing cassette, comprising a gene coding lactate dehydrogenase connected to a site downstream of a promoter, the promoter being a promoter of a gene showing a gene expression amount larger by 5 times or more than the average relative expression amount of all genes after 50 hours from start of culture in continuous culture with simultaneous filtration of a yeast strain having a lactic acid-producing ability.


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