The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 14, 2014

Filed:

May. 29, 2012
Applicants:

Chih-ching Chien, Hsinchu, TW;

Po-chi Soo, Taipei, TW;

Yu-tze Horng, Taipei, TW;

Shan-yu Chen, Chiayi, TW;

Hsiu-hsiung LI, Chung-Li, TW;

Yu-hong Wei, Chung-Li, TW;

Wen-ming Chen, Chung-Li, TW;

Inventors:

Chih-Ching Chien, Hsinchu, TW;

Po-Chi Soo, Taipei, TW;

Yu-Tze Horng, Taipei, TW;

Shan-Yu Chen, Chiayi, TW;

Hsiu-Hsiung Li, Chung-Li, TW;

Yu-Hong Wei, Chung-Li, TW;

Wen-Ming Chen, Chung-Li, TW;

Assignee:

Yuan Ze University, Chung-Li, TW;

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12P 7/62 (2006.01); C12P 19/34 (2006.01); C12N 15/00 (2006.01); C12N 1/20 (2006.01); C07H 21/02 (2006.01);
U.S. Cl.
CPC ...
Abstract

The invention discloses a method for production of polyhydroxybutyrate-co-polyhydroxyvalerate (PHBV) by recombinantharboring plasmid containing both phaCAB and prpE. Different percentage of hydroxyvalerate can be obtained from the recombinantwhen cultivated in the medium containing different concentrations of propionic acid. In this patent, we provide a method that integrated all of the genes (i.e. phaCAB, vgb and prpE) required for PHBV production into a single plasmid. The plasmids were then transformed into anhost. Results showed that PHBV can be produced by this recombinant, and the ration of HV to HB in the co-polymers can be regulated by addition of different concentrations of propionic acid in the medium. The percentage of HV in the co-polymers can be adjusted from about 3% up to more than 35%.


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