The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 07, 2014

Filed:

Aug. 10, 2010
Applicants:

Masayasu Kuwahara, Kiryu, JP;

Tomoharu Kajiyama, Higashiyamato, JP;

Hideki Kambara, Hachioji, JP;

Inventors:

Masayasu Kuwahara, Kiryu, JP;

Tomoharu Kajiyama, Higashiyamato, JP;

Hideki Kambara, Hachioji, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07H 17/00 (2006.01); C07H 21/02 (2006.01); C12Q 1/68 (2006.01); C12P 19/34 (2006.01);
U.S. Cl.
CPC ...
Abstract

Provided is a nucleic acid substrate which has nucleic acid substrate characteristics equivalent to those of dATP, has a low substrate specificity for luciferase, exerts no negative effect on enzymatic reactions such as a complementary-strand synthesis, and therefore is particularly suitable for the pyrosequencing method. As a nucleic acid substrate complementary to nucleotide T, a 7-substituted deoxyribonucleotide triphosphate whose 7-position of a purine group is modified by a substituent is used as a substitute for a nucleotide α-thiotriphosphate analog.


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