The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 07, 2014

Filed:

Jun. 21, 2012
Applicants:

Gabor Forgacs, Columbia, MO (US);

Karoly Jakab, Columbia, MO (US);

Adrian Neagu, Columbia, MO (US);

Vladimir Mironov, Mount Pleasant, SC (US);

Inventors:

Gabor Forgacs, Columbia, MO (US);

Karoly Jakab, Columbia, MO (US);

Adrian Neagu, Columbia, MO (US);

Vladimir Mironov, Mount Pleasant, SC (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 5/07 (2010.01); C12N 11/12 (2006.01); C12N 5/02 (2006.01); B29C 67/00 (2006.01); C12M 1/00 (2006.01); C12M 3/00 (2006.01); C12N 11/00 (2006.01); C12N 5/00 (2006.01); B29L 31/00 (2006.01); B29K 105/00 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0062 (2013.01); B29C 67/0059 (2013.01); C12M 47/00 (2013.01); C12M 21/08 (2013.01); B29L 2031/7532 (2013.01); B29K 2105/0035 (2013.01);
Abstract

A method of making engineered tissue from a plurality of cell aggregates is disclosed. A cell suspension is centrifuged. The resulting pellet is extruded through an orifice, and the extruded pellet is cut into pieces to produce cell aggregates. A plurality of the cell aggregates are printed in a pattern, and allowed to fuse to form a desired three-dimensional engineered tissue structure. Modeling methods predict the structural evolution of fusing cell aggregates for combinations of cell type to enable selection of organ printing process parameters for use in producing an engineered tissue having a desired three-dimensional structure.


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