The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 07, 2014

Filed:

Apr. 21, 2008
Applicants:

Mehmet Toner, Wellesley, MA (US);

Rashid Bashir, Champaign, IL (US);

Xuanhong Cheng, Charlestown, MA (US);

Utkan Demirci, Cambridge, MA (US);

Daniel Irimia, Charlestown, MA (US);

William R. Rodriguez, Cambridge, MA (US);

Liju Yang, Apex, NC (US);

Lee Zamir, Cambridge, MA (US);

Yi-shao Liu, Champaign, IL (US);

Inventors:

Mehmet Toner, Wellesley, MA (US);

Rashid Bashir, Champaign, IL (US);

Xuanhong Cheng, Charlestown, MA (US);

Utkan Demirci, Cambridge, MA (US);

Daniel Irimia, Charlestown, MA (US);

William R. Rodriguez, Cambridge, MA (US);

Liju Yang, Apex, NC (US);

Lee Zamir, Cambridge, MA (US);

Yi-Shao Liu, Champaign, IL (US);

Assignees:

The General Hospital Corporation, Boston, MA (US);

Purdue Research Foundation, West Lafayette, IN (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/567 (2006.01); G01N 33/50 (2006.01); G01N 33/84 (2006.01); G01N 15/10 (2006.01); G01N 15/14 (2006.01);
U.S. Cl.
CPC ...
G01N 33/5005 (2013.01); G01N 15/1056 (2013.01); G01N 2015/1062 (2013.01); G01N 33/84 (2013.01); G01N 2015/1006 (2013.01); G01N 15/1484 (2013.01); G01N 15/1031 (2013.01);
Abstract

The invention features methods of quantifying cells in a sample by lysing the cells followed by the measurement of at least one intracellular component. Methods of the invention are especially useful for quantifying small numbers of cells, e.g., over a large surface area or volume compared to the cell size. In a preferred embodiment, methods of the invention are performed using a microfluidic device.


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