The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 07, 2014

Filed:

Nov. 16, 2005
Applicants:

Betty Wu, Canton, MI (US);

John S. Althaus, Ann Arbor, MI (US);

Nikhil Phadke, Ann Arbor, MI (US);

Sundaresh N. Brahmasandra, Ann Arbor, MI (US);

Kalyan Handique, Ann Arbor, MI (US);

Aaron Kehrer, Ypsilanti, MI (US);

Gene Parunak, Saline, MI (US);

Cecelia Haley, Canton, MI (US);

Ted Springer, Ann Arbor, MI (US);

Inventors:

Betty Wu, Canton, MI (US);

John S. Althaus, Ann Arbor, MI (US);

Nikhil Phadke, Ann Arbor, MI (US);

Sundaresh N. Brahmasandra, Ann Arbor, MI (US);

Kalyan Handique, Ann Arbor, MI (US);

Aaron Kehrer, Ypsilanti, MI (US);

Gene Parunak, Saline, MI (US);

Cecelia Haley, Canton, MI (US);

Ted Springer, Ann Arbor, MI (US);

Assignee:

HandyLab, Inc., Ann Arbor, MI (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2006.01); C12M 1/36 (2006.01); B01L 3/00 (2006.01); B01D 21/00 (2006.01); F16K 99/00 (2006.01); B01L 7/00 (2006.01);
U.S. Cl.
CPC ...
B01L 3/502707 (2013.01); B01L 3/502715 (2013.01); B01L 3/502723 (2013.01); B01L 2200/16 (2013.01); B01L 2300/0867 (2013.01); F16K 99/0019 (2013.01); F16K 99/0001 (2013.01); B01L 2400/0442 (2013.01); B01L 3/502738 (2013.01); B01L 2400/0481 (2013.01); B01L 2300/0816 (2013.01); B01L 7/52 (2013.01); F16K 99/0044 (2013.01); F16K 99/0034 (2013.01); B01L 3/523 (2013.01); B01L 2400/0487 (2013.01); B01L 2400/0633 (2013.01); F16K 2099/0084 (2013.01); B01L 2400/0683 (2013.01); B01L 2300/0672 (2013.01);
Abstract

Methods and systems for processing polynucleotides (e.g., DNA) are disclosed. A processing region includes one or more surfaces (e.g., particle surfaces) modified with ligands that retain polynucleotides under a first set of conditions (e.g., temperature and pH) and release the polynucleotides under a second set of conditions (e.g., higher temperature and/or more basic pH). The processing region can be used to, for example, concentrate polynucleotides of a sample and/or separate inhibitors of amplification reactions from the polynucleotides. Microfluidic devices with a processing region are disclosed.


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