The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 07, 2014
Filed:
Aug. 28, 2009
Randall S. Prather, Rocheport, MO (US);
Rongfeng LI, Hohhot, CN;
Clifton N. Murphy, Columbia, MO (US);
Lee Spate, Columbia, MO (US);
Randall S. Prather, Rocheport, MO (US);
Rongfeng Li, Hohhot, CN;
Clifton N. Murphy, Columbia, MO (US);
Lee Spate, Columbia, MO (US);
The Curators of the University of Missouri, Columbia, MO (US);
Abstract
The present invention provides a practical, non-invasive, and efficient method for cryopreservation of an in-vitro-produced porcine embryo. The inventive method treats the NP (such as IVF- or NT-derived) embryo at the one-cell or cleavage stage prior to compaction with high osmolality followed by high speed centrifugation. The high osmolality treatment enlarges the periviteline space, and with centrifugation, enables the separation of the lipids from the cytoplasm. The lipid-separated embryos after high osmolality treatment have been successfully cryopreserved and later recovered and transferred to produce live offspring.