The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 30, 2014
Filed:
Aug. 07, 2009
Jannick Prento, Bronshoj, DK;
Judith M. Gottwein, Frederiksberg, DK;
Troels Kasper Hoyer Scheel, Copenhagen NV, DK;
Tanja Bertelsen Jensen, Frederiksberg C, DK;
Jens Bukh, Praesto, DK;
Jannick Prento, Bronshoj, DK;
Judith M. Gottwein, Frederiksberg, DK;
Troels Kasper Hoyer Scheel, Copenhagen NV, DK;
Tanja Bertelsen Jensen, Frederiksberg C, DK;
Jens Bukh, Praesto, DK;
Hvidovre Hospital, Hvidovre, DK;
Kobenhavns Universitet, Copenhagen K, DK;
Abstract
The present inventors used the previously developed H77/JFH 1(1a/2a), J4/JFH(1b/2a), J6/JFH(2a/2a), J8/JFH 1(2b/2a), S52/JFH 1(3a/2a), SA13/JFH 1(5a/2a) and HK6a/JFH 1T(6a/2a) constructs for the deletion of Hypervariable Region 1 (HVR1) to construct viable, JFH 1 (genotype 2a) based, genomes. The present inventors serially passaged the viruses in cell culture obtaining relatively high HCV RNA titers and infectivity titers. Sequence analysis of the viruses identified mutations adapting H77/JFH 1(1a/2a), J8/JFH(2b/2a), S52/JFH 1(3a/2a) and J4/JFH 1(1b/2a) to the HVR1 deletion.