The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 23, 2014

Filed:

Jun. 29, 2011
Applicants:

Katsuyuki Dodo, Otsu, JP;

Naoki Saito, Otsu, JP;

Hideto Chono, Otsu, JP;

Junichi Mineno, Otsu, JP;

Inventors:

Katsuyuki Dodo, Otsu, JP;

Naoki Saito, Otsu, JP;

Hideto Chono, Otsu, JP;

Junichi Mineno, Otsu, JP;

Assignee:

Takara Bio Inc., Shiga, JP;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 15/00 (2006.01); C12N 15/867 (2006.01); C12N 15/86 (2006.01); C12N 15/87 (2006.01); C12P 21/04 (2006.01); C12P 21/06 (2006.01);
U.S. Cl.
CPC ...
C12N 15/8676 (2013.01); C12N 15/86 (2013.01); C12N 2740/16043 (2013.01); C12N 15/87 (2013.01);
Abstract

Disclosed is a method for introducing a gene into a target cell using a retrovirus vector, which is simple and highly efficient. Specifically disclosed is a method for introducing a gene into a target cell using a retrovirus vector, which comprises the steps of (a) placing a liquor containing a retrovirus vector having a foreign gene carried thereon into a culture vessel on which a retrovirus-binding substance has been immobilized, and incubating the liquor at a temperature lower than 25° C. for 4 hours or longer, thereby producing a culture vessel having the retrovirus vector bound thereto, and (b) adding a target cell to the culture vessel that has been produced in step (a) and incubating the culture vessel. The gene introduction method is simple and highly efficient, and is useful particularly in the fields of medicine, cell technology, gene technology, embryologic technology and the like.


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