The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 23, 2014

Filed:

Aug. 27, 2009
Applicants:

Ick-chan Kwon, Seoul, KR;

Kui-won Choi, Seoul, KR;

Kwang-meyung Kim, Seoul, KR;

In-chan Youn, Seoul, KR;

Seul-ki Lee, Seoul, KR;

Kyeong-soon Park, Junranam-Do, KR;

Dae-hyuk Moon, Seoul, KR;

Dae-yoon Chi, Seoul, KR;

Seung-jin Lee, Seoul, KR;

Seung-jae Myung, Seoul, KR;

Inventors:

Ick-Chan Kwon, Seoul, KR;

Kui-Won Choi, Seoul, KR;

Kwang-Meyung Kim, Seoul, KR;

In-Chan Youn, Seoul, KR;

Seul-Ki Lee, Seoul, KR;

Kyeong-Soon Park, Junranam-Do, KR;

Dae-Hyuk Moon, Seoul, KR;

Dae-Yoon Chi, Seoul, KR;

Seung-Jin Lee, Seoul, KR;

Seung-Jae Myung, Seoul, KR;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q 1/34 (2006.01); C12Q 1/37 (2006.01); G01N 33/543 (2006.01); C12Q 1/04 (2006.01); C12Q 1/06 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/37 (2013.01); G01N 33/54346 (2013.01); Y10S 977/773 (2013.01); Y10S 977/774 (2013.01); Y10S 977/807 (2013.01); Y10S 977/811 (2013.01); Y10S 977/953 (2013.01);
Abstract

Disclosed are a nanoparticle sensor for measuring protease activity, for protease imaging, and a method for preparing the same. More specifically, the present invention relates to a nanoparticle sensor for measuring protease activity in which a fluorophore- and a quencher-conjugated peptide substrate is conjugated to a biocompatible polymer nanoparticle. The peptide substrate is specifically lysed by a protease. The sensor according to the present invention is capable of inhibiting emission of fluorescence with high extinctive activity of the quencher on a fluorescent material. But strong fluorescence is specifically emitted only if the peptide substrate is lysed by a specific protease. Therefore, the sensor is especially useful as a method for screening a novel drug such as a protease overexpression inhibitor, and early diagnosis of incurable diseases and various diseases such as autoimmune diseases including cancer, osteoarthritis, rheumatoid arthritis and dementia.


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