The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 16, 2014
Filed:
Dec. 20, 2012
Applicant:
John Cuppoletti, Cincinnati, OH (US);
Inventor:
John Cuppoletti, Cincinnati, OH (US);
Assignee:
Other;
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
G01N 33/53 (2006.01); C12M 1/00 (2006.01); C12M 1/34 (2006.01); G01N 33/68 (2006.01); G01N 33/569 (2006.01);
U.S. Cl.
CPC ...
G01N 33/68 (2013.01); G01N 2400/50 (2013.01); G01N 33/56911 (2013.01); G01N 2500/20 (2013.01); Y10S 435/975 (2013.01);
Abstract
A method for determining the amount of live pore forming bacterial toxin protein in a sample is provided, the method including the steps of a) forming a membrane comprising a lipid bilayer and a receptor, b) contacting the membrane with an ion solution and the sample, c) measuring ion flow through the membrane, d) comparing the ion flow through the membrane to a standard curve, and e) determining the amount of pore forming bacterial toxin protein in the sample. A kit for determining the amount of live pore forming bacterial toxin protein present in the sample is also provided.