The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 16, 2014

Filed:

Nov. 01, 2011
Applicants:

Josh Molho, Los Altos, CA (US);

Hui Xu, Palo Alto, CA (US);

Inventors:

Josh Molho, Los Altos, CA (US);

Hui Xu, Palo Alto, CA (US);

Assignee:

Caliper Life Sciences, Inc., Hopkinton, MA (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
B01D 17/06 (2006.01); C12M 1/34 (2006.01); C12M 1/36 (2006.01); G01N 21/05 (2006.01); B01L 3/00 (2006.01); G01N 27/447 (2006.01);
U.S. Cl.
CPC ...
G01N 27/44791 (2013.01); G01N 27/44717 (2013.01); B01L 2400/0655 (2013.01); B01L 2300/0816 (2013.01); B01L 3/502753 (2013.01); B01L 2400/0421 (2013.01); B01L 2200/0636 (2013.01); B01L 3/502738 (2013.01);
Abstract

The invention provides devices and methods for isolating one or more sample components of a sample material following separation of the sample material into a plurality of sample components. A device includes a separation channel having a sample loading well. A low-conductivity buffer is disposed in the loading well, the buffer having a conductivity<0.2 mS/cm. In a method, a buffer is loaded into a loading well in fluid communication with a separation channel of a device. A sample material having a conductivity higher than that of the buffer is then loaded into the loading well such that the sample material is disposed beneath the buffer, the buffer disposed over and covering the sample material. The sample material is separated into a plurality of separated components in the separation channel, and a separated component is collected from a collection well disposed in a collection leg of the device.


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