The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 09, 2014

Filed:

Nov. 10, 2009
Applicants:

Michiya Matsusaki, Osaka, JP;

Mitsuru Akashi, Osaka, JP;

Kazunori Okano, Hyogo, JP;

Inventors:

Michiya Matsusaki, Osaka, JP;

Mitsuru Akashi, Osaka, JP;

Kazunori Okano, Hyogo, JP;

Assignee:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q 1/02 (2006.01); G01N 33/50 (2006.01); G01N 33/543 (2006.01);
U.S. Cl.
CPC ...
G01N 33/5032 (2013.01); G01N 33/5088 (2013.01); G01N 33/54313 (2013.01);
Abstract

Disclosed is a method for detecting a biological signal of a three-dimensional cell culture construct. The method for detecting a biological signal of a three-dimensional cell culture construct includes: providing a three-dimensional cell culture construct that contains at least two cell layers laminated to each other and a sensor particle capable of detecting a biological signal; and observing the sensor particle optically. Preferably, the three-dimensional cell culture construct contains an extracellular matrix including a combination of a protein or polymer having an RGD sequence and a protein or polymer that interacts with the protein or polymer having the RGD sequence, or a combination of a protein or polymer that is positively charged and a protein or polymer that is negatively charged.


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