The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 09, 2014

Filed:

Nov. 16, 2010
Applicants:

Maciej Szczepanik, Mount Sinai, NY (US);

Irina V. Lebedeva, Bronx, NY (US);

Yuejun Xiang, Hollis Hills, NY (US);

Praveen Pande, Holbrook, NY (US);

Wayne Forrest Patton, Dix Hills, NY (US);

Inventors:

Maciej Szczepanik, Mount Sinai, NY (US);

Irina V. Lebedeva, Bronx, NY (US);

Yuejun Xiang, Hollis Hills, NY (US);

Praveen Pande, Holbrook, NY (US);

Wayne Forrest Patton, Dix Hills, NY (US);

Assignee:

Enzo Life Sciences, Inc., Farmingdale, NY (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/26 (2006.01); C07D 311/02 (2006.01); C07D 305/14 (2006.01); C07D 413/00 (2006.01); G01N 21/00 (2006.01);
U.S. Cl.
CPC ...
G09K 11/06 (2013.01); G01N 2333/902 (2013.01); C12Q 1/26 (2013.01); G01N 2500/00 (2013.01);
Abstract

A compound comprising the structure:(SIG)-(SI-MOD).is provided in which SIG is a signaling molecule, SI is a self-immolative structure bound to SIG such that SIG has a reduced signal relative to the signal of SIG without SI, MOD is a moiety bound to SI that is subject to modification by an activator, and m is an integer from 1 to about 10. When MOD is modified by an activator, SI is destabilized and self-cleaved from SIG such that SIG generates an increased signal. Also provided are methods of determining whether a sample comprises an activator such as a nitroreductase, and methods of determining whether a mammalian cell is hypoxic using a compound of the formula (SIG)-(SI-MOD).


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