The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 02, 2014
Filed:
Aug. 09, 2006
Kenji Kinoshita, Hyogo, JP;
Toru Yakabe, Tokyo, JP;
Kentaro Fujimoto, Tokyo, JP;
Kanehisa Yokoyama, Tokyo, JP;
Kazuhiko Fujiwara, Tokyo, JP;
Kenji Kinoshita, Hyogo, JP;
Toru Yakabe, Tokyo, JP;
Kentaro Fujimoto, Tokyo, JP;
Kanehisa Yokoyama, Tokyo, JP;
Kazuhiko Fujiwara, Tokyo, JP;
Sumitomo Bakelite Company, Ltd., Tokyo, JP;
Kenji Kinoshita, Hyogo, JP;
Abstract
A method of synthesizing a cDNA chain using an insoluble carrier having on the surface thereof a polymer substance containing a first unit having a group derived from a phosphoric ester composing the hydrophilic portion of phospholipid, and a second unit having a group derived from carboxylic acid having an electron-attractive substituent bound to a carbonyl group, which includes immobilizing a polynucleotide for DNA elongation; bringing a solution containing an RNA fragment, nucleotide monomers, and a reverse transcriptase or an enzyme having polymerase activity into contact with the surface of the insoluble carrier; and allowing the polynucleotide for DNA elongation immobilized on the surface of the carrier to elongate using the RNA fragment contained in the solution as a template, to thereby form a single-strand cDNA.