The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 26, 2014
Filed:
Jun. 30, 2008
Applicants:
Nathaniel Beardsley, Oregon, WI (US);
Veit Bergendahl, Madison, WI (US);
Megan Fitzgerald, Madison, WI (US);
Christine Daigh, Middleton, WI (US);
Inventors:
Nathaniel Beardsley, Oregon, WI (US);
Veit Bergendahl, Madison, WI (US);
Megan Fitzgerald, Madison, WI (US);
Christine Daigh, Middleton, WI (US);
Assignee:
Cellular Dynamics International, Inc., Madison, WI (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/00 (2006.01); C12N 5/02 (2006.01); C12N 5/071 (2010.01);
U.S. Cl.
CPC ...
Abstract
The invention concerns methods for automated culture of embryonic stem cells (ESCs) such as human ESCs. In some aspects, methods of the invention employ optimized culture media and limited proteolytic treatment of cells to separate cell clusters for expansion. Automated systems for passage and expansion of ESCs are also provided.