The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 26, 2014
Filed:
Mar. 20, 2013
Life Technologies Corporation, Carlsbad, CA (US);
Stephen F. Gorfien, Williamsville, NY (US);
Richard Fike, Clarence, NY (US);
Glenn P. Godwin, Holbrook, MA (US);
Joyce L. Wolanske, Lancaster, NY (US);
David A. Epstein, East Amherst, NY (US);
Dale Gruber, Leesburg, FL (US);
Don McClure, Indianapolis, IN (US);
Paul J. Price, Mount Pleasant, SC (US);
Life Technologies Corporation, Carlsbad, CA (US);
Abstract
The present invention provides a cell culture medium formulation that supports the in vitro cultivation, particularly in suspension, of mammalian cells, particularly epithelial cells and fibroblast cells, and methods for cultivating mammalian cells in suspension in vitro using these media. The present invention also provides chemically defined, protein-free eukaryotic cell culture media comprising an iron chelate and zinc, which is capable of supporting the growth (and particularly the high-density growth of mammalian cells) in suspension culture, increasing the level of expression of recombinant protein in cultured cells, and/or increasing virus production in cultured cells. The invention also provides kits for use in the cultivation of a mammalian epithelial cell that comprise one or more containers, wherein a first container contains the culture medium of the invention. These kits may further comprise one or more additional containers containing one or more supplements.