The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 19, 2014
Filed:
May. 09, 2012
Kenneth J. Livak, San Jose, CA (US);
Stacey N. Meyers, San Francisco, CA (US);
Jun Wang, Palo Alto, CA (US);
Xiaohui Wang, Foster City, CA (US);
Kenneth J. Livak, San Jose, CA (US);
Stacey N. Meyers, San Francisco, CA (US);
Jun Wang, Palo Alto, CA (US);
Xiaohui Wang, Foster City, CA (US);
Fluidigm Corporation, South San Francisco, CA (US);
Abstract
The invention provides a method for detecting a target nucleotide sequence by tagging the nucleotide sequence with a nucleotide tag, providing a probe oligonucleotide with a melting temperature Tm, comprising a regulatory sequence and a nucleotide tag recognition sequence; incorporating the probe oligonucleotide into the tagged polynucleotide in a polynucleotide amplification reaction, providing a regulatory oligonucleotide with a melting temperature Tm, comprising a sequence segment that is at least partially complementary to the regulatory sequence, amplifying the tagged target nucleic acid sequence in a PCR amplification reaction using the probe oligonucleotide as a primer, and detecting the amplification product; wherein Tmand Tmare higher than the annealing temperature associated with the polynucleotide amplification reaction.