The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 12, 2014
Filed:
Jul. 31, 2013
3m Innovative Properties Company, St. Paul, MN (US);
Stephen B. Roscoe, Woodbury, MN (US);
Phillip A. Bolea, Grant, MN (US);
Stephanie J. Moeller, Stillwater, MN (US);
3M Innovative Properties Company, St. Paul, MN (US);
Abstract
A system for detecting microorganisms in a test sample is provided that includes a fluorescently detectable product having both acidic and basic species, wherein the fluorescently detectable product is the reaction product of (a) an enzyme substrate that comprises an enzymatically hydrolysable group and a fluorescent group and (b) a test sample comprising a microorganism having an enzyme that hydrolyzes the hydrolysable group from the fluorescent group of the enzyme substrate. The fluorescently detectable product has an excitation isosbestic point Exλiso where the absorbance of the acid species is the same as the absorbance of the basic species. The system also includes a first light source having a wavelength of Exλiso for irradiating the fluorescently detectable product, The fluorescently detectable product emits light at a wavelength of Emλ1, and the quantity of light emitted at the wavelength Emλ1 is substantially constant across a pH range of 2.5 to 8.0.