The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 05, 2014
Filed:
Feb. 18, 2010
Michael Seul, Fanwood, NJ (US);
Yi Zhang, Hillsborough, NJ (US);
Sukanta Banerjee, Pennington, NJ (US);
Jiacheng Yang, Hillsborough, NJ (US);
Chiu Chau, Edison, NJ (US);
Michael Seul, Fanwood, NJ (US);
Yi Zhang, Hillsborough, NJ (US);
Sukanta Banerjee, Pennington, NJ (US);
Jiacheng Yang, Hillsborough, NJ (US);
Chiu Chau, Edison, NJ (US);
BioArray Solutions, Ltd., Warren, NJ (US);
Abstract
In a multiplexed assay method carried out in solution, wherein the solution contains nucleic acid targets and, wherein several different types of oligonucleotide probes, each type having a different sequence in a region designated as a target binding domain, are used to detect the nucleic acid targets, said assay method including a method for increasing the effective concentration of the nucleic acid targets at the surface of a bead to which the oligonucleotide probes are bound, by one or more of the following steps: adjusting assay conditions so as to increase the effective concentration of the targets available for binding to the probes, by one or more of the following: (i) selecting a particular probe density on the surface of the bead; (ii) selecting a solution having an ionic strength greater than a threshold; (ii) selecting a target domain of a size less than a threshold; or (iii) selecting target domains within a specified proximity to a terminal end of the targets.