The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 05, 2014
Filed:
Jun. 08, 2009
Michael Seul, Fanwood, NJ (US);
Sukanta Banerjee, Pennington, NJ (US);
Jiacheng Yang, Hillsboro, NJ (US);
Tatiana Vener, Stirling, NJ (US);
Michael Seul, Fanwood, NJ (US);
Sukanta Banerjee, Pennington, NJ (US);
Jiacheng Yang, Hillsboro, NJ (US);
Tatiana Vener, Stirling, NJ (US);
Bioarray Solutions, Ltd., Warren, NJ (US);
Abstract
Disclosed are methods of multiplexed analysis of oligonucleotides in a sample, including: methods of probe and target 'engineering', as well as methods of assay signal analysis relating to the modulation of the probe-target affinity constant, K by a variety of factors including the elastic properties of target strands and layers of immobilized ('grafted') probes; and assay methodologies relating to: the tuning of assay signal intensities including dynamic range compression and on-chip signal amplification; the combination of hybridization-mediated and elongation-mediated detection for the quantitative determination of abundance of messages displaying a high degree of sequence similarity, including, for example, the simultaneous determination of the relative expression levels, and identification of the specific class of, untranslated AU-rich subsequences located near the 3' terminus of mRNA; and a new method of subtractive differential gene expression analysis which requires only a single color label.