The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 29, 2014
Filed:
Apr. 03, 2012
Blas Cerda, Milford, MA (US);
Mark Norman Bobrow, Lexington, MA (US);
Blas Cerda, Milford, MA (US);
Mark Norman Bobrow, Lexington, MA (US);
PerkinElmer Health Sciences, Inc., Waltham, MA (US);
Abstract
An inventive substrate is provided which includes a substrate compound of formula A-B-B-B-B: wherein A is a sugar moiety; Bis a linker moiety allowing the conjugation of moiety A and the remaining structure of the substrate; Bis a linker moiety with a free reactive amino group so as to be available for reaction with carboxylic acids or detectable tags; Bcontains a permanently charged element such as a quaternary ammonium group so as to increase sensitivity for mass spectrometry analysis; and Bof various carbon length conferring specificity amongst individual substrates in detection methods. Also provided is a molecule of the formula B-B-B-B, with similar structural characteristics to an enzymatic product produced by the action of a target enzyme on an inventive substrate. Further provided are methods for using inventive substrates for detecting enzymatic activity.