The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 8785169 B1

PDF
Full Text
Expired
Date of Patent:
Jul. 22, 2014

Filed:

Feb. 28, 2011
Applicants:

Jian Chen, Wuxi, CN;

Jing Wu, Wuxi, CN;

Dan Wu, Wuxi, CN;

Lei Wang, Wuxi, CN;

Inventors:

Jian Chen, Wuxi, CN;

Jing Wu, Wuxi, CN;

Dan Wu, Wuxi, CN;

Lei Wang, Wuxi, CN;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/18 (2006.01); C12N 1/20 (2006.01);
U.S. Cl.
CPC ...
C12N 9/18 (2013.01); C12Y 301/01074 (2013.01);
Abstract

The present invention relates to the field of bioengineering and discloses a cutinase-producing genetically engineered microorganism and use thereof. Recombinant plasmid Tfu_0883-hlyAs/pET20b(+) was constructed and transformed intoBL21(DE3) to obtain recombinantstrain Tfu_0883-hlyAs/pET20b(+)/BL21(DE3). Specific growth rate was maintained at a certain value using fed-batch fermentation mode. After fermenting 30-34 hours, the enzyme activity in the supernatant reached 700-750 U/mL. The present invention uses glycerol as the main raw material and employs semi-synthetic medium, has the advantages of good stability and ease of control, and is suitable for large-scale production.


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Chen, Jian. (2014). Cutinase-producing genetically engineered microorganism and use thereof (U.S. Patent No. 8785169). U.S. Patent and Trademark Office. https://idiyas.com/patent/badge/8785169

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