The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 15, 2014
Filed:
May. 22, 2012
Aldis Darzins, Verona, WI (US);
Lance Encell, Fitchburg, WI (US);
Tonny Johnson, Madison, WI (US);
Dieter Klaubert, Arroyo Grande, CA (US);
Georgyi V. Los, Madison, WI (US);
Mark Mcdougall, Arroyo Grande, CA (US);
Keith V. Wood, Mt. Horeb, WI (US);
Monika G. Wood, Mt. Horeb, WI (US);
Chad Zimprich, Stoughton, WI (US);
Aldis Darzins, Verona, WI (US);
Lance Encell, Fitchburg, WI (US);
Tonny Johnson, Madison, WI (US);
Dieter Klaubert, Arroyo Grande, CA (US);
Georgyi V. Los, Madison, WI (US);
Mark McDougall, Arroyo Grande, CA (US);
Keith V. Wood, Mt. Horeb, WI (US);
Monika G. Wood, Mt. Horeb, WI (US);
Chad Zimprich, Stoughton, WI (US);
Promega Corporation, Madison, WI (US);
Abstract
A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.