The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 08, 2014
Filed:
Oct. 05, 2009
Judith M. Gottwein, Frederiksberg C, DK;
Troels Kasper Hoyer Scheel, Copenhagen, DK;
Jens Bukh, Praesto, DK;
Jannick Prento, Bronshoj, DK;
Tanja Bertelsen Jensen, Frederiksberg C, DK;
Jacob BO Lademann, Copenhagen S, DK;
Yiping LI, Hvidovre, DK;
Judith M. Gottwein, Frederiksberg C, DK;
Troels Kasper Hoyer Scheel, Copenhagen, DK;
Jens Bukh, Praesto, DK;
Jannick Prento, Bronshoj, DK;
Tanja Bertelsen Jensen, Frederiksberg C, DK;
Jacob Bo Lademann, Copenhagen S, DK;
Yiping Li, Hvidovre, DK;
Hvidovre Hospital, Hvidovre, DK;
Kobenhavns Universitet, Copenhagen K, DK;
Abstract
Hepatitis C reporter viruses containing Core through NS2 of prototype isolates of all major HCV genotypes and the remaining genes of isolate JFH1, by insertion of reporter genes in domain III of HCV NS5A were developed. A deletion upstream of the inserted reporter gene sequence conferred favorable growth kinetics in Huh7.5 cells to these viruses. These reporter viruses can be used for high throughput analysis of drug and vaccine candidates as well as patient samples. JFH1-based intergenotypic recombinants with genotype specific homotypic 5'UTR, or heterotypic 5′UTR (either of genotype 1a (strain H77) or of genotype 3a (strain S52)) were also developed. The present inventors additionally developed J6/JFH1 recombinants with the 5′UTR of genotypes 1-6. These recombinants with different 5′UTRs are a useful to study the function of the 5′UTR in a genotype specific manner.