The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 01, 2014
Filed:
Jul. 22, 2011
Applicants:
Tzu-pin Wang, Tainan, TW;
Yi-jhang Ciou, Pingtung County, TW;
Inventors:
Tzu-Pin Wang, Tainan, TW;
Yi-Jhang Ciou, Pingtung County, TW;
Assignee:
Kaohsiung Medical University, Kaohsiung, TW;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07H 21/00 (2006.01); C12Q 1/68 (2006.01); C07D 293/10 (2006.01); C07D 235/02 (2006.01);
U.S. Cl.
CPC ...
Abstract
A method for conjugating a nucleic acid with a molecule is provided. The method includes steps of (a) reacting the nucleic acid having a 5'-monophosphate with an activating agent in a first buffer to form a solution; (b) mixing an alcohol with the solution formed in the step (a) to obtain an intermediate; and (c) dissolving the intermediate in a second buffer containing an ethylenediaminetetraacetic acid (EDTA) and adding a nucleophile thereinto to react the intermediate with the nucleophile.