The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 01, 2014
Filed:
Nov. 17, 2010
Alahari Arunakumari, Pennington, NJ (US);
Xiao-ping Dai, Flemington, NJ (US);
Javier Garcia, Palmerton, NJ (US);
Richard P. Martel, Allentown, PA (US);
Alahari Arunakumari, Pennington, NJ (US);
Xiao-Ping Dai, Flemington, NJ (US);
Javier Garcia, Palmerton, NJ (US);
Richard P. Martel, Allentown, PA (US);
Medarex, L.L.C., Princeton, NJ (US);
Abstract
The present invention provides a method of increasing protein production in a cell culture by growing cells that produce the protein (e.g., the growth phase) in a perfusion cell culture to a high cell density (i.e., at least above about 40×10cells/N mL) and then switching to a protein production phase, wherein the cells are cultured in a fed-batch cell culture. The present invention further provides a method for clarifying a protein from a cell culture by adjusting the pH of the cell culture to below neutral pH (i.e., below a pH of 7) and settling the cell culture, such that the cell culture separates to form a supernatant layer and a cell-bed layer, wherein the protein is in the supernatant layer.