The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 24, 2014

Filed:

May. 10, 2012
Applicants:

Shiaw-min Hwang, Taipei, TW;

Chi-hsien Liu, Taipei, TW;

Chao-ling Yao, Taipei, TW;

I-ming Chu, Taipei, TW;

Tzu-bou Hsieh, Taipei, TW;

Inventors:

Shiaw-Min Hwang, Taipei, TW;

Chi-Hsien Liu, Taipei, TW;

Chao-Ling Yao, Taipei, TW;

I-Ming Chu, Taipei, TW;

Tzu-Bou Hsieh, Taipei, TW;

Assignee:
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/48 (2006.01); G01N 33/50 (2006.01); G01N 31/00 (2006.01); G06F 7/60 (2006.01); G06F 17/10 (2006.01); G06F 19/24 (2011.01); G06F 19/12 (2011.01); G06F 19/28 (2011.01); G06F 17/15 (2006.01); G06F 17/11 (2006.01);
U.S. Cl.
CPC ...
G06F 19/24 (2013.01); G06F 19/12 (2013.01); G06F 19/28 (2013.01); G06F 17/15 (2013.01); G06F 17/11 (2013.01);
Abstract

The present invention provides a method of determining the optimal composition of a serum-free, eukaryotic cell culture medium supplement, using 2-level factorial design and the steepest ascent method. The invention further provides a method of making a serum-free eukaryotic cell culture medium supplement and the generated thereof. The invention further provides a method of making a serum-free, eukaryotic cell culture medium and the medium generated thereof. The invention further provides a kit containing the medium of the invention. The invention also provides a method of expanding CD34<+> hematopoietic cells and a composition comprising CD34<+> hematopoietic cells in a serum-free, eukaryotic cell culture medium of the invention.


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