The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 24, 2014

Filed:

Mar. 23, 2006
Applicants:

Masamichi Takagi, Fuchu, JP;

Takahisa Kogure, Kashiwa, JP;

Naoki Wakisaka, Niigata, JP;

Hiroaki Takaku, Niigata, JP;

Katsumi Ajisaka, Niigata, JP;

Tatsuo Miyazaki, Niigata, JP;

Masao Hirayama, Niigata, JP;

Inventors:

Masamichi Takagi, Fuchu, JP;

Takahisa Kogure, Kashiwa, JP;

Naoki Wakisaka, Niigata, JP;

Hiroaki Takaku, Niigata, JP;

Katsumi Ajisaka, Niigata, JP;

Tatsuo Miyazaki, Niigata, JP;

Masao Hirayama, Niigata, JP;

Assignees:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
A01N 63/00 (2006.01); C12N 15/09 (2006.01); C12N 15/63 (2006.01); C07H 21/04 (2006.01);
U.S. Cl.
CPC ...
Abstract

A transformant is prepared to insert at least a gene expression cassette comprising a gene involved in the synthesis of 2-deoxy-scyllo-inosose intoas host cells. A 2-deoxy-scyllo-inosose is synthesized from D-glucose, oligosaccharide, polysaccharide, starch and rice bran, using the transformant. A culture solution containing the 2-deoxy-scyllo-inosose is treated with a mixed bed or double bed type column comprising a hydrogen form of strong acidic cation exchange resin and an organic ion form of basic anion exchange resin. The 2-deoxy-scyllo-inosose as purified is reacted with trimethoxymethane to convert into 2-deoxy-scyllo-inosose dimethylketal, and the dimethylketal is crystallized and purified. Then, DOI is highly purified through hydrolyzing the dimethylketal in the presence of acid.


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