The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 10, 2014
Filed:
Feb. 05, 2009
Jean-marc Nicaud, Trappes, FR;
Franck Fudalej, Rueil-Malmaison, FR;
Cécile Neuveglise, Auffargis, FR;
Jean-marie Beckerich, Bourg-la-Reine, FR;
Jean-Marc Nicaud, Trappes, FR;
Franck Fudalej, Rueil-Malmaison, FR;
Cécile Neuveglise, Auffargis, FR;
Jean-Marie Beckerich, Bourg-la-Reine, FR;
Institut National de la Recherche Agronomique, Paris, FR;
Abstract
This invention concerns a method for the targeted integration of at least three copies of a gene of interest in the genome of astrain including the steps of: (a) cultivating astrain, said strain including a deletion among at least three genes, the phenotype associated with each of these deletions corresponding to an auxotrophy or to a dominant character for this strain; (b) transforming saidstrain thus obtained with at least three recombinant vectors that include selection markers allowing, for this strain, the complementation of auxotrophy and, potentially, of the dominant character resulting from each of these deletions; and (c) selecting, on a minimum medium, the yeasts having integrated said at least three recombinant vectors. This invention also includes a method for producing a polypeptide of interest using this method as well as a method for obtaining a modifiedstrain including a deletion among at least three genes, the phenotype associated with each of these deletions corresponding to an auxotrophy or to a dominant character for this strain.