The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 10, 2014
Filed:
Dec. 17, 2010
Gregory Timp, South Bend, IN (US);
Winston Timp, Baltimore, MD (US);
Utkur Mirsaidov, Urbana, IL (US);
Aleksei Aksimentiev, Urbana, IL (US);
Jeffrey Comer, Urbana, IL (US);
Gregory Timp, South Bend, IN (US);
Winston Timp, Baltimore, MD (US);
Utkur Mirsaidov, Urbana, IL (US);
Aleksei Aksimentiev, Urbana, IL (US);
Jeffrey Comer, Urbana, IL (US);
The Board of Trustees of the University of Illinois, Urbana, IL (US);
The John Hopkins University, Baltimore, MD (US);
Abstract
Methods of trapping a deformed portion of a double-stranded polynucleotide in a membrane nanopassage are provided. In an aspect, the membrane has a nanopassage that defines a confine region, wherein the membrane separates a first fluid compartment from a second fluid compartment, and the nanopassage is in fluid communication with the first and second compartments. A polynucleotide is provided to the first fluid compartment and optionally a threshold voltage for the membrane and the polynucleotide is determined. A driving voltage across the membrane that is greater than the threshold voltage is applied to force a portion of the polynucleotide sequence into the nanopassage confine region, and decreased to a holding voltage bias to trap the polynucleotide portion in the nanopassage confine region. In particular, at least one nucleotide base-pair is fixably positioned in the nanopassage confine volume. In further embodiments, any of the trapping methods are used to characterize or sequence double stranded DNA.