The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 03, 2014

Filed:

Nov. 13, 2009
Applicants:

Yoshikazu Yonemitsu, Chiba, JP;

Yasuji Ueda, Ibaraki, JP;

Yui Harada, Saitama, JP;

Inventors:

Yoshikazu Yonemitsu, Chiba, JP;

Yasuji Ueda, Ibaraki, JP;

Yui Harada, Saitama, JP;

Assignee:

DNAVEC Corporation, Tsukuba-shi, Ibaraki, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
A61K 39/00 (2006.01); C12N 5/0784 (2010.01);
U.S. Cl.
CPC ...
C12N 5/0639 (2013.01); C12N 2501/125 (2013.01); C12N 2501/22 (2013.01); C12N 2501/23 (2013.01); A61K 39/00 (2013.01);
Abstract

An objective of the present invention is to provide methods for producing dendritic cells (DCs), which comprise the step of culturing DC precursor cells in the presence of a plurality of cytokines, produced dendritic cells, and uses thereof. The present inventors discovered that dendritic cells with a high IL-12 productivity can be obtained by culturing DC precursor cells in the presence of a plurality of cytokines, followed by about one week of culture in the presence of GM-CSF and IL-4. The present invention enables preparation of a large amount of DCs with a high IL-12 productivity from a small number of DC precursor cells, and therefore makes it easier to increase the number of DCs for administration in DC-based anti-tumor immune therapy, treatment of infections, etc. Thus, the effect of DC vaccines is expected to be enhanced.


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