The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 03, 2014
Filed:
Apr. 26, 2012
Bruce Seligmann, Tucson, AZ (US);
Debrah Thompson, Tucson, AZ (US);
Tom Vasicek, Minneapolis, MN (US);
Debra A. Gordon, Tucson, AZ (US);
Bruce Seligmann, Tucson, AZ (US);
Debrah Thompson, Tucson, AZ (US);
Tom Vasicek, Minneapolis, MN (US);
Debra A. Gordon, Tucson, AZ (US);
HTG Molecular Diagnostics, Inc., Tucson, AZ (US);
Abstract
The present disclosure provides an improvement to quantitative Nuclease Protection Assay (qNPA) and quantitative Nuclease Protection Sequencing (qNPS) methods. The disclosed methods use nuclease protection probes (NPPs) that include 5'-end and/or 3-end flanking sequences, which provide a universal hybridization and/or amplification sequence. The disclosed methods can be used to sequence or detect target nucleic acid molecules, such as those present in fixed or insoluble samples.