The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 27, 2014
Filed:
Aug. 12, 2009
Ryota Hashizume, Uji, JP;
Bunzo Mikami, Uji, JP;
Hirotaka Matsubara, Kakamigahara, JP;
Akiko Matsunaga, Kakamigahara, JP;
Shotaro Yamaguchi, Kakamigahara, JP;
Ryota Hashizume, Uji, JP;
Bunzo Mikami, Uji, JP;
Hirotaka Matsubara, Kakamigahara, JP;
Akiko Matsunaga, Kakamigahara, JP;
Shotaro Yamaguchi, Kakamigahara, JP;
Amano Enzyme Inc., Nagoya-shi, JP;
Abstract
An object is to provide a novel method for improving an enzyme capable of deamidating a protein. A mutant protein deamidase is designed by the following steps: (A) identifying one or more amino acid in an amino acid sequence for a protein deamidase which corresponds to the amino acid at position 35, 38 to 43, 45, 46, 49, 79 to 84, 103 to 106, 117, 142, 143, 146, 166, or 185 in the amino acid sequence set forth in SEQ ID NO: 2; and (B) constructing a mutant amino acid sequence of the protein deamidase by substituting the one or more amino acid identified in step (A) with another amino acid or other amino acids or by deleting the one or more amino acid identified in step (A).