The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 20, 2014
Filed:
Feb. 20, 2009
Marcello Maresca, Arlington, MA (US);
Axel Steffen Erler, Haselbach, DE;
Jun Fu, Dresden, DE;
Philipp Martin Seibert, Dresden, DE;
Adrian Francis Stewart, Dresden, DE;
Youming Zhang, Saarbrucken, DE;
Marcello Maresca, Arlington, MA (US);
Axel Steffen Erler, Haselbach, DE;
Jun Fu, Dresden, DE;
Philipp Martin Seibert, Dresden, DE;
Adrian Francis Stewart, Dresden, DE;
Youming Zhang, Saarbrucken, DE;
Gene Bridges GmbH, , DE;
Abstract
The invention provides a method for inserting a single stranded replacement nucleic acid into a target nucleic acid, the method comprising the steps of: a) generating a single stranded replacement nucleic acid from a double stranded nucleic acid, wherein the double stranded nucleic acid is adapted at one or both of its 5' ends such that preferential degradation of one strand and/or strand separation generates the single stranded replacement nucleic acid, wherein the single stranded replacement nucleic acid comprises a 5′ region that is identical to sequence on the target nucleic acid, a 3′ region that is identical to sequence on the target nucleic acid and optionally a replacement region between the 5′ and 3′ regions that is not identical to sequence on the target nucleic acid, b) exposing the target nucleic acid to the single stranded replacement nucleic acid under conditions suitable for recombination to occur between the single stranded replacement nucleic acid and the target nucleic acid, and c) selecting a target nucleic acid whose sequence has been altered by inclusion of said single stranded replacement nucleic acid. Other methods for modifying target nucleic acids are also provided.