The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 20, 2014
Filed:
Feb. 15, 2012
Jesus Ching, San Jose, CA (US);
Ronald Chang, Redwood City, CA (US);
Douglas Dority, Mill Valley, CA (US);
Jian Ping Zhang, Moraga, CA (US);
James Jian Quan Wang, Fremont, CA (US);
Wendy Wong, Mountain View, CA (US);
Kendra Lara Paul, Santa Clara, CA (US);
Reuel Van Atta, Palo Alto, CA (US);
Jesus Ching, San Jose, CA (US);
Ronald Chang, Redwood City, CA (US);
Douglas Dority, Mill Valley, CA (US);
Jian Ping Zhang, Moraga, CA (US);
James Jian Quan Wang, Fremont, CA (US);
Wendy Wong, Mountain View, CA (US);
Kendra Lara Paul, Santa Clara, CA (US);
Reuel Van Atta, Palo Alto, CA (US);
Cepheid, Sunnyvale, CA (US);
Abstract
The invention is directed to systems, methods, and apparatus for carrying out multi-stage amplification reactions, especially under fluidly closed conditions. In one aspect, methods of the invention are carried out in a fluidly closed reaction system that permits the isolation of a portion of a first (or prior) reaction mixture and its use as a sample or specimen in a second (or subsequent) reaction mixture, thereby substantially avoiding interfering effects that first reaction components may have in the second reaction if both reaction mixtures were simply combined together. In this aspect, systems, methods, and apparatus of the invention may be used with any amplification reaction that permits multiple stages of amplification based on the use of nested primers.