The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 06, 2014
Filed:
Apr. 19, 2012
Liu Xiaohai, Nr Saffron Walden, GB;
John Milton, Nr Saffron Walden, GB;
Geoffrey Paul Smith, Nr Saffron Walden, GB;
Colin Lloyd Barnes, Trumpington, GB;
Isabelle Marie Julia Rasolonjatovo, Nr Saffron Walden, GB;
Roberto Rigatti, Nr Saffron Walden, GB;
Xiaolin Wu, Nr Saffron Walden, GB;
Tobias William Barr Ost, Nr Saffron Walden, GB;
Graham John Worsley, Nr Saffron Walden, GB;
David James Earnshaw, Nr Saffron Walden, GB;
Gerardo Turcatti, Geneva, CH;
Anthony Romieu, Rouen, FR;
Liu Xiaohai, Nr Saffron Walden, GB;
John Milton, Nr Saffron Walden, GB;
Geoffrey Paul Smith, Nr Saffron Walden, GB;
Colin Lloyd Barnes, Trumpington, GB;
Isabelle Marie Julia Rasolonjatovo, Nr Saffron Walden, GB;
Roberto Rigatti, Nr Saffron Walden, GB;
Xiaolin Wu, Nr Saffron Walden, GB;
Tobias William Barr Ost, Nr Saffron Walden, GB;
Graham John Worsley, Nr Saffron Walden, GB;
David James Earnshaw, Nr Saffron Walden, GB;
Gerardo Turcatti, Geneva, CH;
Anthony Romieu, Rouen, FR;
Illumina Cambridge Limited, Nr Saffron Walden, GB;
Abstract
The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to a solid support at the 5' end, cleaving one or both strands of the double-stranded nucleic acid molecule, and subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction.