The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 15, 2014
Filed:
Dec. 14, 2009
Masamitsu Shikata, Kyoto, JP;
Masamitsu Shikata, Kyoto, JP;
Shimadzu Corporation, Kyoto-shi, JP;
Abstract
A DNA amplification method including: subjecting a blood sample having DNA to be amplified, to a pretreatment using an alkaline aqueous solution under ordinary temperature, so as to extract double-stranded DNA from the blood sample and dissociate the double-stranded DNA into a single-stranded DNA to obtain a blood-derived sample including the single-stranded DNA; preparing an isothermal amplification reaction solution comprising a mixture of the blood-derived sample, a primer, dNTP, a strand-displacing DNA polymerase, a magnesium salt and a buffer, to establish an isothermal amplification reaction system meeting optimum conditions for the strand-displacing DNA polymerase; and amplifying DNA in the isothermal amplification reaction system using the single-stranded DNA as a template.