The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 15, 2014

Filed:

Jul. 14, 2009
Applicants:

Mette Elena Skindersø, Virum, DK;

Helle Frobøse Sørensen, Copenhagen V, DK;

Søren Kjaerulff, Hillerod, DK;

Inventors:

Mette Elena Skindersø, Virum, DK;

Helle Frobøse Sørensen, Copenhagen V, DK;

Søren Kjaerulff, Hillerod, DK;

Assignee:

ChemoMetec A/S, Allerød, DK;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q 1/02 (2006.01);
U.S. Cl.
CPC ...
Abstract

The present invention provides simple, rapid methods and procedures for analyzing cells, hereunder quantitative and qualitative assessment of cells. The present invention relates to the use of N-(9-acridinyl)maleimide (NAM) or to the use of 7-diethylamino-3-((4'-(iodoacetyl)amino)phenyl)-4-methylcoumarin (CPI), particularly detectable upon its reaction with species (e.g., sulphur-containing species) present in higher concentrations in intact (e.g., living) cells than in non-intact (e.g., dead) cells. The present invention also relates to the use of NAM or to the use of 7-diethylamino-3-((4′-(iodoacetyl)amino)phenyl)-4-methylcoumarin (CPI), particularly detectable upon its reaction with species present in intact and/or non-intact cells. Moreover, the present invention relates to the use of measuring techniques and/or instruments coupled with the use of NAM or with the use of 7-diethylamino-3-((4′-(iodoacetyl)amino)phenyl)-4-methylcoumarin (CPI). The invention further relates to compositions used in methods for analyzing cells.


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